High performance liquid chromatographic determination of nicotine and cotinine in plasma and nicotine and cotinine, simultaneously, in urine

Three analytical procedures were developed to determine nicotine in plasma, cotinine in plasma and, simultaneously, nicotine and cotinine in urine. After liquid or solid-phase extraction, the purified aqueous phase is injected into a high performance liquid chromatograph equipped with an ultra-viole...

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Bibliographic Details
Published inMethods and findings in experimental and clinical pharmacology Vol. 11; no. 3; p. 179
Main Authors Sioufi, A, Parisot, C, Sandrenan, N, Dubois, J P
Format Journal Article
LanguageEnglish
Published Spain 01.03.1989
Subjects
Amphetamine - analysis
Amphetamine - blood
Amphetamine - urine
Chromatography, Gas
Chromatography, High Pressure Liquid
Cotinine - analysis
Cotinine - blood
Cotinine - urine
Humans
Isoquinolines - analysis
Isoquinolines - blood
Isoquinolines - urine
Nicotine - analysis
Nicotine - blood
Nicotine - urine
Pyrrolidinones - analysis
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Summary:Three analytical procedures were developed to determine nicotine in plasma, cotinine in plasma and, simultaneously, nicotine and cotinine in urine. After liquid or solid-phase extraction, the purified aqueous phase is injected into a high performance liquid chromatograph equipped with an ultra-violet detector using a CN Spheri-5 micron cartridge-column with an inner diameter of 4.6 mm and a length of 10 or 22 cm. The limit of quantitation for nicotine in plasma was around 8 to 15 ng/ml, that of cotinine in plasma around 50 ng/ml and that of nicotine and cotinine in urine around 170 ng/ml and 70 ng/ml, respectively. The limit of detection of nicotine in plasma was around 1 ng/ml and that of nicotine and cotinine in urine around 20 ng/ml and 10 ng/ml, respectively. The passive exposure to cigarette smoke by non-smokers and the "resting levels" of nicotine in plasma and urine of smokers were studied. The analytical methods were set up to study the pharmacokinetics and bioavailability of nicotine in healthy volunteers following single and repeated administrations of different doses of transdermal nicotine systems.
ISSN:0379-0355