Specificity of the isolectins from the plant cactus Machaerocereus eruca for oligosaccharides from porcine stomach mucin

Sugar specificity of the Machaerocereus eruca isolectins, MeAI and MeAII, has been determined by comparing the capacity of glycans with well defined structures to inhibit their haemagglutinating activity. Both are galactose-specific isolectins with high affinity for O-glycans. However, the two M. er...

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Published inGlycoconjugate journal Vol. 12; no. 5; pp. 699 - 706
Main Authors Zenteno, E, Vázquez, L, Chávez, R, Córdoba, F, Wieruszeski, J M, Montreuil, J, Debray, H
Format Journal Article
LanguageEnglish
Published United States 01.10.1995
Subjects
Animals
Carbohydrate Sequence
Chromatography, Gel
Erythrocytes - immunology
Hemagglutination
Humans
Indicators and Reagents
Lectins - chemistry
Lectins - isolation & purification
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Monosaccharides - analysis
Mucins - chemistry
Oligosaccharides - chemistry
Oligosaccharides - isolation & purification
Plant Lectins
Stomach
Substrate Specificity
Swine
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Summary:Sugar specificity of the Machaerocereus eruca isolectins, MeAI and MeAII, has been determined by comparing the capacity of glycans with well defined structures to inhibit their haemagglutinating activity. Both are galactose-specific isolectins with high affinity for O-glycans. However, the two M. eruca isolectins recognize different oligosaccharidic sequences belonging to O-glycosidically linked glycans from porcine stomach mucin. The minimal structure recognized by MeAI on the porcine mucin glycans is the O-glycan core Gal beta 1,3GalNAc-ol, whereas MeAII has a more extended site and interacts with a biantennary O-glycan possessing the terminal trisaccharide Fuc alpha 1,2 (GalNAc alpha 1,3) Gal beta 1,4.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0282-0080
DOI:10.1007/BF00731267