An equation for the reconstitution of membrane proteins and a method for determination of pore content in biological membranes

The dependence between specific trapped volume of liposomes (W) and protein concentration (p) is proposed to be used for quantitative pore determination in biological membranes via pore reconstitution into liposomes. This dependence is described by the following equation: p = -p(e) x ln(W/W0), where...

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Published inBiochemistry (Moscow) Vol. 63; no. 11; pp. 1328 - 1334
Main Authors Lagutina, L S, Sholtz, P K, Mamaev, D V, Sholtz, K F
Format Journal Article
LanguageEnglish
Published United States 01.11.1998
Subjects
Animals
Computer Simulation
Intracellular Membranes - metabolism
Kinetics
Liposomes - chemistry
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Mitochondria - metabolism
Models, Biological
Porins - metabolism
Rats
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Summary:The dependence between specific trapped volume of liposomes (W) and protein concentration (p) is proposed to be used for quantitative pore determination in biological membranes via pore reconstitution into liposomes. This dependence is described by the following equation: p = -p(e) x ln(W/W0), where W0 is initial trapped volume of liposomes and pe is an equivalent protein concentration at which molar concentrations of pores and liposomes become equal. Experimentally determined equivalent protein concentration pe is the basis of the method. This method also permits determination of molar mass of pore-forming complexes provided that preparations contain purified complex.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0006-2979