Differentiation of gram-positive and gram-negative bacteria and yeasts using a modification of the "string" test

Solutions of potassium hydroxide and sodium hydroxide with concentrations between 0.5% and 4% were found to produce mucoid and sticky suspensions when added in drops, using a bacteriological loop, to smears of 609 strains of gram-negative bacteria on glass slides. A total of 461 strains of gram-posi...

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Bibliographic Details
Published inThe American journal of medical technology Vol. 49; no. 3; p. 177
Main Authors Agbonlahor, D E, Odugbemi, T O, Udofia, P O
Format Journal Article
LanguageEnglish
Published United States 01.03.1983
Subjects
Bacteria - isolation & purification
Hydroxides
Microbiological Techniques
Potassium
Potassium Compounds
Sodium Hydroxide
Yeasts - isolation & purification
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Summary:Solutions of potassium hydroxide and sodium hydroxide with concentrations between 0.5% and 4% were found to produce mucoid and sticky suspensions when added in drops, using a bacteriological loop, to smears of 609 strains of gram-negative bacteria on glass slides. A total of 461 strains of gram-positive bacteria and yeasts did not produce any mucoid or "string" formation when treated with the same solutions. It was concluded that this modified test could aid in differentiating gram-positive from gram-negative organisms.
ISSN:0002-9335