Flow cytometric reticulocyte quantification using thiazole orange provides clinically useful reticulocyte maturity index

Flow cytometric reticulocyte quantification with thiazole orange has been reported to be of potential utility in a clinical hematology laboratory. We have instituted this technique into routine clinical testing for 18 months and we describe this experience. Flow cytometric analysis provided not only...

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Published inArchives of pathology & laboratory medicine (1976) Vol. 113; no. 6; pp. 684 - 689
Main Authors Davis, B H, Bigelow, N C
Format Journal Article
LanguageEnglish
Published United States 01.06.1989
Subjects
Benzothiazoles
Bone Marrow Transplantation
Cell Count
Cell Survival
Cost-Benefit Analysis
Erythropoiesis
Flow Cytometry - economics
Flow Cytometry - methods
Flow Cytometry - standards
Fluorescent Dyes
Humans
Quality Control
Quinolines
Reticulocytes - analysis
Reticulocytes - cytology
Reticulocytes - physiology
RNA - analysis
Thiazoles
Transplantation, Autologous
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Summary:Flow cytometric reticulocyte quantification with thiazole orange has been reported to be of potential utility in a clinical hematology laboratory. We have instituted this technique into routine clinical testing for 18 months and we describe this experience. Flow cytometric analysis provided not only reproducible, cost-effective reticulocyte quantification, but a quantitative reticulocyte maturity index proportional to the amount of RNA in the reticulocytes. The reticulocyte maturity index measurement represents an independent parameter of erythropoiesis, which provided clinically valuable information regarding bone marrow engraftment in patients following autologous bone marrow transplantation. The findings of this study demonstrate the clinical utility of thiazole orange reticulocyte analysis and indicate the diagnostic importance of the reticulocyte maturity index measurement in the evaluation of erythropoietic activity.
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ISSN:0003-9985