Changes in blood and bronchoalveolar lavage fluid components in calves with experimentally induced pneumonic pasteurellosis

Pneumonic pasteurellosis was experimentally induced in calves by inoculation of 5 x 10(8) Pasteurella haemolytica organisms into the right diaphragmatic lung lobe. Blood and bronchoalveolar lavage fluid samples were obtained prior to inoculation and at postinoculation hour (PIH) 2, 4, and 6. Calves...

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Published inAmerican journal of veterinary research Vol. 52; no. 2; p. 337
Main Authors Weiss, D J, Bauer, M C, Whiteley, L O, Maheswaran, S K, Ames, T R
Format Journal Article
LanguageEnglish
Published United States 01.02.1991
Subjects
Animals
Animals, Newborn - blood
Bronchoalveolar Lavage Fluid - chemistry
Bronchoalveolar Lavage Fluid - veterinary
Cattle
Cattle Diseases - blood
Cattle Diseases - etiology
Iron Chelating Agents - pharmacology
Leukocyte Count - veterinary
Male
Pasteurellosis, Pneumonic - blood
Pasteurellosis, Pneumonic - etiology
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Summary:Pneumonic pasteurellosis was experimentally induced in calves by inoculation of 5 x 10(8) Pasteurella haemolytica organisms into the right diaphragmatic lung lobe. Blood and bronchoalveolar lavage fluid samples were obtained prior to inoculation and at postinoculation hour (PIH) 2, 4, and 6. Calves developed acute lung injury, characteristic of pneumonic pasteurellosis. Lesions were found only in the right diaphragmatic lobe. By PIH 4, significant (P less than 0.01) increases were detected in lavage fluid total cell count, neutrophil count, total protein and albumin concentrations, and alkaline phosphatase (ALP) and lactic dehydrogenase (LD) activities. Myeloperoxidase and elastase activities did not increase. Neutrophil depletion ameliorated the lung lesions and prevented the increase in lavage fluid cell count, total protein, and albumin concentrations and ALP and LD activities. Treatment with the iron chelator, deferoxamine mesylatehydroxyethyl starch, attenuated the increase in total protein and albumin concentrations and ALP and LD activities at PID 4, but not PIH 6. Treatment with a neutrophil function inhibitor, pentoxifylline, prevented the increase in lavage fluid neutrophil numbers, but accentuated the increase in total protein and albumin concentrations, and ALP, LD, myeloperoxidase, and elastase activities.
ISSN:0002-9645