Protein antigens of genetically related Rickettsia prowazekii strains with different virulence

• Published in: Acta virologica (Anglickâa verze) Vol. 36; no. 1; p. 52
• Main Authors: Balayeva, N M, Eremeeva, M E, Ignatovich, V F, Dmitriev, B A, Lapina, E B, Belousova, L S
• Format: Journal Article
• Language: English
• Published: Slovakia 01.01.1992
• Subjects: Animals; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Chick Embryo; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Rabbits; Rickettsia prowazekii - genetics; Rickettsia prowazekii - immunology; Rickettsia prowazekii - pathogenicity; Typhus, Epidemic Louse-Borne - immunology; Typhus, Epidemic Louse-Borne - microbiology; Virulence
• ISSN: 0001-723X

The protein antigens of two distinct lines of genetically related strains, namely the nonpathogenic strain E and its virulent revertant EVir and of the standard virulent strain Breinl were compared in SDS-PAGE and immunoblot assay using typhus patient sera and immune rabbit sera. No differences in the polypeptide pattern as detected in SDS-PAGE were found between strain E and EVir; the Breinl strain differed in a 30 kD protein. The high immunogenicity of the protein antigens of E, EVir and Breinl strains was demonstrated by immunoblot assay with human sera, which did not show any differences between the strains studied. Immunoblot analysis with immune rabbit sera to the strain E, EVir, and Breinl showed differences in immunological response to the 70 kD and 60 kD polypeptides of low virulent strain E and those of virulent strains EVir and Breinl.