The complete nucleotide sequence of the gene coding for botulinum type C1 toxin in the C-ST phage genome

Two DNA fragments, 3 kbp and 7.8kbp, which encode the type C1 botulinum neurotoxin gene, were obtained from toxigenic bacteriophage DNA by treatment with a restriction enzyme. They were cloned into the plasmid vectors for nucleotide sequence determination. The nucleotide sequence contained a single...

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Published inBiochemical and biophysical research communications Vol. 171; no. 3; p. 1304
Main Authors Kimura, K, Fujii, N, Tsuzuki, K, Murakami, T, Indoh, T, Yokosawa, N, Takeshi, K, Syuto, B, Oguma, K
Format Journal Article
LanguageEnglish
Published United States 28.09.1990
Subjects
Amino Acid Sequence
Bacteriophages - genetics
Base Sequence
Botulinum Toxins - genetics
Chromosome Mapping
Cloning, Molecular - methods
Clostridium botulinum - genetics
DNA, Viral - genetics
DNA, Viral - isolation & purification
Genes, Bacterial
Genes, Viral
Molecular Sequence Data
Plasmids
Polymerase Chain Reaction - methods
Sequence Homology, Nucleic Acid
Viral Structural Proteins - genetics
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Summary:Two DNA fragments, 3 kbp and 7.8kbp, which encode the type C1 botulinum neurotoxin gene, were obtained from toxigenic bacteriophage DNA by treatment with a restriction enzyme. They were cloned into the plasmid vectors for nucleotide sequence determination. The nucleotide sequence contained a single open reading frame coding for 1,291 amino acids corresponding to a polypeptide with a molecular weight of 149,000. The amino acid sequence of the C1 toxin has a few regions highly homologous with tetanus toxin.
ISSN:0006-291X
DOI:10.1016/0006-291X(90)90828-B