Comparison of the protein composition of Erysipelothrix rhusiopathiae strains of subtype 1A isolated from ducks and pigs

Erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (SDS-PAGE) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular ma...

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Bibliographic Details
Published inActa veterinaria Hungarica (Budapest. 1983) Vol. 46; no. 2; p. 211
Main Authors Bernáth, S, Morovján, G, Sztojkov, V, Szita, G
Format Journal Article
LanguageEnglish
Published Hungary 1998
Subjects
Animals
Bacterial Proteins - chemistry
Densitometry - veterinary
Ducks - microbiology
Electrophoresis, Polyacrylamide Gel - veterinary
Erysipelothrix - chemistry
Erysipelothrix Infections - epidemiology
Erysipelothrix Infections - microbiology
Molecular Weight
Poultry Diseases - epidemiology
Poultry Diseases - microbiology
Reproducibility of Results
Statistics, Nonparametric
Swine - microbiology
Swine Diseases - epidemiology
Swine Diseases - microbiology
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Summary:Erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (SDS-PAGE) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. To characterise the similarity of the strains, Pearson's correlation coefficient was calculated. The degree of similarity between duck strains originating from the same flock was 980-991, while that of strains isolated from ducks and pigs varied between 836 and 991 on a scale of 0 to 1000. The method gives highly reproducible results and can be utilised in epidemiological investigations. Its use may provide new data on E. rhusiopathiae strains, complementing the results of methods recommended for this purpose earlier and increasing the efficiency of epidemiological investigations.
ISSN:0236-6290