Application of a novel chemiluminescence-based DNA detection method to single-vector and multiplex DNA sequencing

A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reactio...

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Bibliographic Details
Published inBioTechniques Vol. 11; no. 1; p. 102
Main Authors Creasey, A, D'Angio, Jr, L, Dunne, T S, Kissinger, C, O'Keeffe, T, Perry-O'Keefe, H, Moran, L S, Roskey, M, Schildkraut, I, Sears, L E
Format Journal Article
LanguageEnglish
Published England 01.07.1991
Subjects
Base Sequence
DNA - chemistry
DNA-Directed DNA Polymerase - metabolism
Electrophoresis, Polyacrylamide Gel
Genetic Techniques
Genetic Vectors - genetics
Luminescent Measurements
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Summary:A chemiluminescent DNA detection method is described and its application shown for both single-vector and multiplex DNA sequencing using the standard dideoxy chain-termination process. This recently developed detection method, which utilizes the light emitted by an enzyme-catalyzed dioxetane reaction, is highly sensitive and affords significant advantages in safety and speed over the traditional radioactive labeling method. When adapted to a multiplex strategy, this chemiluminescent detection method constitutes a safe, simple and rapid method for increasing the throughput of DNA sequencing procedures.
ISSN:0736-6205