Glycosphingolipids in murine lymphocytes from thymus and spontaneous and X-ray induced thymomas

Lymphocytes from spontaneous thymoma in AKR mice and from X-ray induced thymoma in C57B1/6 mice showed elevated levels (by 50% and 100%, respectively) of lipid-bound sialic acid as compared with lymphocytes from normal thymuses used as controls. Some ganglioside fractions in thymomas were elevated 4...

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Bibliographic Details
Published inActa biochimica polonica Vol. 32; no. 1; p. 47
Main Authors Bartoszewicz, Z, Lipska, M, Pacuszka, T, Rossowski, W
Format Journal Article
LanguageEnglish
Published Poland 1985
Subjects
Animals
Glycosphingolipids - analysis
Mice
Mice, Inbred Strains
Neoplasms, Radiation-Induced - analysis
T-Lymphocytes - analysis
Thymoma - analysis
Thymoma - etiology
Thymus Gland - analysis
X-Rays
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Summary:Lymphocytes from spontaneous thymoma in AKR mice and from X-ray induced thymoma in C57B1/6 mice showed elevated levels (by 50% and 100%, respectively) of lipid-bound sialic acid as compared with lymphocytes from normal thymuses used as controls. Some ganglioside fractions in thymomas were elevated 4-6-fold over those in normal thymuses while other fractions decreased or disappeared. Neutral glycosphingolipid (NGSL) content in lymphocytes from thymomas was also changed. Thin-layer chromatography of NGSLs showed that the fractions migrating as ceramide monohexoside (CMH), dihexoside (CDH) and below globoside standards were increased, respectively, 2-3-fold, 3-6-fold and 2-fold in both types of thymomas. Methylation and gas-liquid chromatography analysis confirmed the presence of CMH, CDH and globoside in NGSLs isolated from X-ray induced thymoma.
ISSN:0001-527X