Enzyme activities in blood cells of man and dogs after separation on a discontinuous Percoll gradient

From human and dog peripheral blood, thrombocytes (TRC), mononuclear cells (MNC), polymorphonuclear cells (PMNC), and red blood cells (RBC), were harvested after separation on a discontinuous Percoll gradient in a two-step centrifugation procedure, and 12 enzyme activities were determined in these h...

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Bibliographic Details
Published inEnzyme (Basel) Vol. 29; no. 2; p. 100
Main Authors Lindena, J, Sommerfeld, U, Höpfel, C, Wolkersdorfer, R, Trautschold, I
Format Journal Article
LanguageEnglish
Published Switzerland 1983
Subjects
Animals
Blood Platelets - cytology
Blood Platelets - enzymology
Cell Separation - methods
Centrifugation, Zonal - methods
Colloids
Dogs
Enzymes - blood
Erythrocytes - cytology
Erythrocytes - enzymology
Humans
Male
Monocytes - cytology
Monocytes - enzymology
Neutrophils - cytology
Neutrophils - enzymology
Povidone
Silicon Dioxide
Species Specificity
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Summary:From human and dog peripheral blood, thrombocytes (TRC), mononuclear cells (MNC), polymorphonuclear cells (PMNC), and red blood cells (RBC), were harvested after separation on a discontinuous Percoll gradient in a two-step centrifugation procedure, and 12 enzyme activities were determined in these highly purified cells. The enzyme activities measured are generally severalfold higher than previously reported, a fact which is attributed to the gentle and time-shortening isolation in Percoll, the cell disruption technique using detergent and the enzyme test conditions.
ISSN:0013-9432
DOI:10.1159/000469614