Anticoagulant-free prepared blood platelets and the effect of calcium on their aggregation behaviour

Human blood platelets were prepared by gel filtration of native blood and subsequent centrifugation in a Ficoll density gradient avoiding any anticoagulant during the preparation. In ADP, ionophore A 23 187 and thrombin induced aggregation an increase in extracellular Ca++ enhanced the velocity of a...

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Bibliographic Details
Published inArtery Vol. 8; no. 5; p. 437
Main Authors Hofmann, B, Lösche, W, Hofmann, J, Till, U, Thielmann, K
Format Journal Article
LanguageEnglish
Published United States 1980
Subjects
Adenosine Diphosphate - pharmacology
Anticoagulants
Blood Platelets
Calcimycin - pharmacology
Calcium - pharmacology
Cell Separation
Humans
Platelet Aggregation - drug effects
Thrombin - pharmacology
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Summary:Human blood platelets were prepared by gel filtration of native blood and subsequent centrifugation in a Ficoll density gradient avoiding any anticoagulant during the preparation. In ADP, ionophore A 23 187 and thrombin induced aggregation an increase in extracellular Ca++ enhanced the velocity of aggregation. From a defined Ca++ concentration threshold on there was a shift from monophasic to biphasic aggregation.
ISSN:0098-6127