Microtubule affinity chromatography: a new technique for isolating microtubule-binding proteins from rat pancreas

We have developed an affinity chromatography method for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts of rat pancreas. Among the ten proteins which copurify with pancreas tubulin on a colchicine derivatives-affinity chromatography, three polypeptides of re...

Full description

Saved in:
Bibliographic Details
Published inCellular and molecular biology Vol. 37; no. 8; p. 805
Main Authors Michalik, L, Vanier, M T, Launay, J F
Format Journal Article
LanguageEnglish
Published England 1991
Subjects
Animals
Chromatography, Affinity - methods
Colchicine - metabolism
Microtubule-Associated Proteins - isolation & purification
Microtubules - metabolism
Pancreas - chemistry
Rats
tau Proteins
Online AccessGet more information

Cover

More Information
Summary:We have developed an affinity chromatography method for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts of rat pancreas. Among the ten proteins which copurify with pancreas tubulin on a colchicine derivatives-affinity chromatography, three polypeptides of respectively 58, 55 and 48 kDa strongly bind to the microtubule affinity column. To begin to characterize these proteins, we have generated polyclonal antibodies against tau polypeptides from brains of immature chicken or rat. As judged by immunoblots, the three polypeptides seem to be immunologically related to the tau proteins previously localized in brain.