Modeling of irreversible thermal protein denaturation at varying temperature. I. The model involving two consecutive irreversible steps

The model of thermal protein denaturation involving two consecutive irreversible steps has been analyzed. The equation describing the dependence of excess heat capacity (Cpex) on temperature (T) under a constant scanning rate has been obtained and used for construction of theoretical Cpex versus T p...

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Bibliographic Details
Published inBiochemistry (Moscow) Vol. 63; no. 4; pp. 434 - 440
Main Authors Lyubarev, A E, Kurganov, B I
Format Journal Article
LanguageEnglish
Published United States 01.04.1998
Subjects
Animals
Calorimetry, Differential Scanning
Humans
Models, Chemical
Models, Theoretical
Protein Denaturation
Proteins - chemistry
Temperature
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Summary:The model of thermal protein denaturation involving two consecutive irreversible steps has been analyzed. The equation describing the dependence of excess heat capacity (Cpex) on temperature (T) under a constant scanning rate has been obtained and used for construction of theoretical Cpex versus T profiles. The change in the shape of theoretical profiles at varied Arrhenius equation parameters, ratio for enthalpies of the first and second steps, and the scanning rate have been studied. It has been shown that formal use of one-step model does not provide adequate information about kinetic features of the process in the case of protein denaturation following the mechanism involving two consecutive irreversible steps.
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SourceType-Scholarly Journals-1
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content type line 23
ISSN:0006-2979