Comparative analysis of TGF beta s, BMPs, IGF1, msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation

• Published in: The International journal of developmental biology Vol. 38; no. 3; pp. 405 - 420
• Main Authors: Bègue-Kirn, C, Smith, A J, Loriot, M, Kupferle, C, Ruch, J V, Lesot, H
• Format: Journal Article
• Language: English
• Published: Spain 01.09.1994
• Subjects: Animals; Animals, Newborn; Bone Morphogenetic Proteins; Cell Differentiation - drug effects; Cell Polarity - drug effects; Culture Techniques; Dental Papilla - metabolism; DNA-Binding Proteins - biosynthesis; DNA-Binding Proteins - genetics; Extracellular Matrix - metabolism; Gene Expression Regulation, Developmental; Glycoproteins - biosynthesis; Glycoproteins - genetics; Heparin - pharmacology; Homeodomain Proteins - biosynthesis; Homeodomain Proteins - genetics; In Situ Hybridization; Insulin-Like Growth Factor I - biosynthesis; Insulin-Like Growth Factor I - genetics; Insulin-Like Growth Factor I - pharmacology; Mice; MSX1 Transcription Factor; Odontoblasts - cytology; Odontoblasts - physiology; Protein Biosynthesis; Proteins - genetics; Proteins - pharmacology; RNA, Messenger - biosynthesis; Transcription Factors; Transforming Growth Factor beta - biosynthesis; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - pharmacology
• ISSN: 0214-6282; 1696-3547

Immobilized TGF beta 1 and BMP2 are able to promote the differentiation of odontoblast-like cells in isolated mouse dental papillae cultured in vitro. These cells polarize and accumulate predentin-like matrix at their apical pole. Immobilized IGF1 mainly promoted polarization with disturbed matrix accumulation. In situ hybridization demonstrated that TGF beta 1 combined with heparin mirrored the physiological processes of odontoblast differentiation. Normal odontoblast and in vitro induced odontoblast-like cells expressed transcripts encoding for TGF beta 1 and 3, BMP2 and 4, bone sialoprotein and osteonectin whereas either ubiquitous expression or no expression could be detected for TGF beta 2, IGF1 or fibronectin mRNAs. Odontoblast-like cells obtained in the presence of IGF-1 combined with heparin did not express TGF beta 1 transcripts and expressed weakly TGF beta 3 transcripts. Our results suggest that in vivo an epithelial-derived member of the TGF beta family trapped by basement membrane-associated components interacts with competent preodontoblasts and promotes the polarization by triggering the transcription of growth factor gene(s) like TGF beta itself and/or selector gene(s) like msx2.