Age-dependent and tissue-specific changes of DNA methylation within and around the c-fos gene in mice

The degree of DNA methylation in the c-fos gene and its vicinity in liver, brain and spleen of mice of different ages was examined using methylation-sensitive restriction endonucleases. The gene had an invariable unmethylated domain from 1.8kb upstream of the cap site to the first intron. The domain...

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Bibliographic Details
Published inOncogene Vol. 4; no. 8; p. 1023
Main Authors Uehara, Y, Ono, T, Kurishita, A, Kokuryu, H, Okada, S
Format Journal Article
LanguageEnglish
Published England 01.08.1989
Subjects
Aging
Animals
Brain - physiology
DNA - genetics
Gene Expression Regulation
Liver - physiology
Methylation
Mice
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-fos
Proto-Oncogenes
Restriction Mapping
Spleen - physiology
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Summary:The degree of DNA methylation in the c-fos gene and its vicinity in liver, brain and spleen of mice of different ages was examined using methylation-sensitive restriction endonucleases. The gene had an invariable unmethylated domain from 1.8kb upstream of the cap site to the first intron. The domain was flanked on both sides with partially methylated areas, where tissue-specificity and age-dependent changes were observed. The tissue-specificity was found on the 14th day of gestation. Increases of methylation were observed during the late fetal and one month postnatal developmental periods in the three tissues. After that, the methylation states remained unchanged. One exception was seen in the exon 2 area in liver, where methylation continued to increase throughout life. This revealed the importance of the late fetal- and post-natal developmental periods in the establishment of tissue-specificity in DNA methylation profiles and further showed that some of the developmental changes could extend into the senescent phase.
ISSN:0950-9232
1476-5594