Surface morphology and ultrastructure of normal and cyclic hematopoietic canine bone marrow in long-term liquid cultures

Long-term liquid cultures of normal and cyclic hematopoietic (CH) dog bone marrow produce committed granulocyte-macrophage progenitor cells (CFU-GM) and differentiated granulocytes for several weeks. Analysis of in situ fixed cultures or of cells harvested from the culture supernatants revealed that...

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Bibliographic Details
Published inHistology and histopathology Vol. 4; no. 3; p. 335
Main Authors al-Lebban, Z S, Lange, R D, Jones, J B, Lothrop, Jr, C D
Format Journal Article
LanguageEnglish
Published Spain 01.07.1989
Subjects
Animals
Bone Marrow - ultrastructure
Cell Adhesion
Cell Nucleus - ultrastructure
Cells, Cultured
Culture Techniques
Dogs
Hematopoietic Stem Cells - ultrastructure
Macrophages - ultrastructure
Microscopy, Electron, Scanning
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Summary:Long-term liquid cultures of normal and cyclic hematopoietic (CH) dog bone marrow produce committed granulocyte-macrophage progenitor cells (CFU-GM) and differentiated granulocytes for several weeks. Analysis of in situ fixed cultures or of cells harvested from the culture supernatants revealed that the cells had ultrastructure and surface morphology characteristic of immature and mature myeloid cells. The surface morphologies of adherent cells from both normal and CH dogs were similar. The characteristic abnormalities previously reported in neutrophils obtained from CH dogs were not observed in neutrophils obtained from long-term marrow cultures of CH dogs. These results indicate that the cellular abnormalities in the neutrophils of CH dogs may be secondary manifestations of the disease and are not inherent to the pathogenesis of the hematopoietic cells.
ISSN:0213-3911