Purification of peanut agglutinin by affinity chromatography on asialoglycophorin from human erythrocytes

A simple and efficient method of purification of peanut agglutinin is described. This method involves affinity chromatography on human asialoglycophorin linked to Affi-Gel. One milliliter of the packed adsorbent contained 1.7 mg of covalently linked asialoglycophorin and bound about 6 mg of the pean...

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Bibliographic Details
Published inArchivum Immunologiae et Therapiae Experimentalis Vol. 31; no. 2; p. 127
Main Authors Starościk, K, Waśniowska, K, Syper, D, Lisowska, E
Format Journal Article
LanguageEnglish
Published Switzerland 1983
Subjects
Asialoglycoproteins
Chromatography, Affinity
Erythrocytes
Glycoproteins
Humans
Lectins - isolation & purification
Peanut Agglutinin
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Summary:A simple and efficient method of purification of peanut agglutinin is described. This method involves affinity chromatography on human asialoglycophorin linked to Affi-Gel. One milliliter of the packed adsorbent contained 1.7 mg of covalently linked asialoglycophorin and bound about 6 mg of the peanut agglutinin. From 250 g of row peanuts 510 mg of the electrophoretically homogeneous lectin with high agglutinating activity was obtained.
ISSN:0004-069X