Evidence that renin substrate (angiotensinogen) may be a precursor of erythropoietin

We suggested a role for renin substrate (RS, angiotensinogen) in the biogenesis of erythropoietin (EP) (Nature 308:649-652, 1984). Purified RS was prepared from pooled plasma of healthy adult subjects, or from plasma of pregnant, healthy females. Antisera against human RS were shown to cross-react w...

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Published inScandinavian journal of urology and nephrology. Supplement Vol. 90; p. 41
Main Authors Fyhrquist, F, Rosenlöf, K, Grönhagen-Riska, C, Hortling, L, Tikkanen, I
Format Journal Article
LanguageEnglish
Published England 1985
Subjects
Adult
Angiotensinogen - analysis
Angiotensinogen - physiology
Angiotensins - physiology
Cerebellar Neoplasms - analysis
Cross Reactions
Erythropoietin - analysis
Erythropoietin - biosynthesis
Erythropoietin - urine
Female
Fluorescent Antibody Technique
Hemangiosarcoma - analysis
Humans
Immunoenzyme Techniques
Pregnancy
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Summary:We suggested a role for renin substrate (RS, angiotensinogen) in the biogenesis of erythropoietin (EP) (Nature 308:649-652, 1984). Purified RS was prepared from pooled plasma of healthy adult subjects, or from plasma of pregnant, healthy females. Antisera against human RS were shown to cross-react with purified human urinary EP, using a haemagglutination technique. On the other hand, antisera generated against highly purified human urinary EP showed strong cross-reaction with purified human plasma RS, by agglutinating erythrocytes covered with glutaraldehyde-coupled human RS. Moreover, using antisera generated against RS or EP, we have demonstrated monocytoid cells staining positive for both EP and RS in cerebellar haemangioblastoma tumors from 8 patients. Taken together, these observations point to a close relation between RS and EP. One interesting explanation is that renin substrate may be a precursor of erythropoietin.
ISSN:0300-8886
1651-2537