Depression of prolylendopeptidase activity in the delayed hypersensitive guinea pig skin lesion induced by bovine gamma-globulin

Prolylendopeptidase activity was increasingly depressed with time from 6 to 24 hr after the start of sensitization in the delayed hypersensitive guinea pig skin lesion induced by bovine gamma-globulin as an antigen. The remarkably depressed activity of the enzyme in the violently inflamed skin began...

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Bibliographic Details
Published inBiochemistry international Vol. 18; no. 6; p. 1183
Main Authors Shoji, S, Imazumi, K, Yamaoka, T, Funakoshi, T, Tanaka, J, Kambara, T, Ueki, H, Kubota, Y
Format Journal Article
LanguageEnglish
Published Australia 01.06.1989
Subjects
Amino Acids - analysis
Animals
Cattle
Chromatography, High Pressure Liquid
Depression, Chemical
Electrophoresis, Polyacrylamide Gel
Endopeptidases - metabolism
gamma-Globulins
Guinea Pigs
Hypersensitivity, Delayed - enzymology
Male
Protease Inhibitors
Serine Endopeptidases
Time Factors
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Summary:Prolylendopeptidase activity was increasingly depressed with time from 6 to 24 hr after the start of sensitization in the delayed hypersensitive guinea pig skin lesion induced by bovine gamma-globulin as an antigen. The remarkably depressed activity of the enzyme in the violently inflamed skin began to be restored slowly 48 hr after sensitization, and its activity was ultimately recovered to the original level by 504 hr after a single sensitization in vivo. Depression of the enzymatic activity is caused by a novel prolyendopeptidase inhibitor, whose amino acid composition is 7 Glu, 1 Ser, 2 Gly, 1 Ala, 2 Pro, and 1 Val, generated by inflammation.
ISSN:0158-5231