The demonstration of acetylcholinesterase in plastic sections. Its application as a marker of early neuronal development

In this paper a histotechnical procedure with respect to the demonstration of acetylcholinesterase in Technovit 7100 (a recently developed GMA) sections is described. From fixation up to embedding several procedural steps were varied and tested, resulting in a suitable procedure. The embedded tissue...

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Bibliographic Details
Published inActa histochemica. Supplementband Vol. 32; p. 185
Main Authors van Straaten, H W, Hekking, J W, Drukker, J
Format Journal Article
LanguageEnglish
Published Germany 1986
Subjects
Acetylcholinesterase - analysis
Acetylcholinesterase - physiology
Animals
Chick Embryo
Fixatives
Microtomy - methods
Neurons - enzymology
Polyhydroxyethyl Methacrylate
Polymethacrylic Acids
Temperature
Time Factors
Tissue Preservation
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ISSN0567-7556

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Summary:In this paper a histotechnical procedure with respect to the demonstration of acetylcholinesterase in Technovit 7100 (a recently developed GMA) sections is described. From fixation up to embedding several procedural steps were varied and tested, resulting in a suitable procedure. The embedded tissue or sections can be stored for months at 7 degrees C with only a slight reduction of the enzymic activity. The enzyme also appears relatively temperature resistant and independent of section thickness (2-10 microns). The enzymic activity remains constant during incubation. The technique has been applied to our subject of study in order to qualify and quantify several aspects of the development of neuroblasts.
ISSN:0567-7556