Characterization of Gardnerella vaginalis by gas chromatography

The principal objective of this study was the characterization of Gardnerella vaginalis by gas chromatography. Thirty-eight isolates and the type strain, ATCC 14018, of G. vaginalis were studied. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids detected. On...

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Bibliographic Details
Published inScandinavian journal of infectious diseases. Supplementum Vol. 40; p. 19
Main Authors Csángó, P A, Hagen, N, Jagars, G
Format Journal Article
LanguageEnglish
Published England 1983
Subjects
Agar
Animals
Blood
Chromatography, Gas - methods
Fatty Acids - analysis
Female
Gardnerella vaginalis - analysis
Gardnerella vaginalis - growth & development
Gardnerella vaginalis - isolation & purification
Haemophilus - analysis
Haemophilus Infections - microbiology
Hemolysis
Humans
In Vitro Techniques
Sheep
Vaginitis - microbiology
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Summary:The principal objective of this study was the characterization of Gardnerella vaginalis by gas chromatography. Thirty-eight isolates and the type strain, ATCC 14018, of G. vaginalis were studied. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids detected. Only insignificant differences between the various isolates could be found. The gas chromatographic analysis of G. vaginalis revealed a characteristic pattern. Gas chromatography in combination with selective growth conditions provides a method for rapid and exact identification.
ISSN:0300-8878