Cytoskeletal reorganization induced by nerve growth factor and glia maturation factor in anaplastic glioma cells

Changes in cytoskeletal organization in T9 anaplastic glioma cells have been examined during morphological changes induced by nerve growth factor (NGF) and by glia maturation factor (GMF). Indirect immunofluorescent labeling of cytoskeletal proteins has revealed that while neither GMF nor NGF induce...

Full description

Saved in:
Bibliographic Details
Published inAnticancer research Vol. 9; no. 4; p. 1143
Main Authors Marushige, Y, Marushige, K, Okazaki, D L, Koestner, A
Format Journal Article
LanguageEnglish
Published Greece 01.07.1989
Subjects
Actins - analysis
Animals
Cell Line
Cytoskeletal Proteins - analysis
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Fluorescent Antibody Technique
Glia Maturation Factor
Glioma - pathology
Growth Substances - pharmacology
Nerve Growth Factors - pharmacology
Nerve Tissue Proteins - pharmacology
Rats
Tubulin - analysis
Tumor Cells, Cultured - cytology
Tumor Cells, Cultured - drug effects
Vimentin - analysis
Online AccessGet more information

Cover

More Information
Summary:Changes in cytoskeletal organization in T9 anaplastic glioma cells have been examined during morphological changes induced by nerve growth factor (NGF) and by glia maturation factor (GMF). Indirect immunofluorescent labeling of cytoskeletal proteins has revealed that while neither GMF nor NGF induces expression of glial fibrillary acidic protein in this cell line, changes in cytoskeletal organization induced by these factors show some features similar to those observable during maturation of normal glial cells. Changing cell shapes induced by these factors are clearly outlined by the prominent distribution of microfilaments along cellular margins. Microtubules and intermediate filaments gradually extend during morphological changes and fill the characteristic cytoplasmic processes induced by NGF and GMF.
ISSN:0250-7005