Effect of denervation and phentolamine on the thermogenic action of noradrenaline in rat skeletal muscle

Vascularly isolated skeletal muscle of the cold-acclimated (CA) rat was perfused with blood in situ or in vitro and the effect of denervation and an alpha-adrenolytic agent (phentolamine) on its oxygen consumption was studied in the resting state and after administering noradrenaline (NA). The resti...

Full description

Saved in:
Bibliographic Details
Published inPhysiologia Bohemoslovaca Vol. 30; no. 2; p. 123
Main Authors Kolár, F, Mejsnar, J
Format Journal Article
LanguageEnglish
Published Czech Republic 1981
Subjects
Animals
Denervation
Hindlimb
In Vitro Techniques
Male
Muscles - blood supply
Muscles - metabolism
Norepinephrine - pharmacology
Oxygen Consumption - drug effects
Phentolamine - pharmacology
Rats
Regional Blood Flow
Online AccessGet more information

Cover

More Information
Summary:Vascularly isolated skeletal muscle of the cold-acclimated (CA) rat was perfused with blood in situ or in vitro and the effect of denervation and an alpha-adrenolytic agent (phentolamine) on its oxygen consumption was studied in the resting state and after administering noradrenaline (NA). The resting metabolism of muscle in situ rose by 28% after denervation. The infusion of NA further raised the oxygen consumption of acutely denervated muscle perfused in situ of in vitro by 43%. The thermogenic effect of NA on muscle denervated two hours before the experiment was only transitory. Phentolamine raised the oxygen consumption of the innervated muscle in situ by 42%; the infusion of NA did not stimulate metabolism any further. Phentolamine reduced the vascular resistance of resting muscle, but did not inhibit the vasoconstriction during the infusion of NA. The results show that the thermogenic effect of infused NA in perfused muscle is inhibited not by acute denervation, but by a vasoconstriction, which cannot be prevented by the administration of an alpha-adrenolytic agent.
ISSN:0369-9463