Proteolytic activity of subcellular fractions from Streptomyces griseus no. 45-H

Subcellular fractions were prepared from Streptomyces griseus No. 45-H at different stages of life cycle, and their proteolytic activity was examined. The highest proteolytic activity was found in the 24- and 72- h-old vegetative hyphae, the lowest in the resting spores. Spores contained about 9--30...

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Bibliographic Details
Published inActa biologica Academiae Scientiarum Hungaricae Vol. 27; no. 2-3; p. 171
Main Authors Valu, G, Szabó, G
Format Journal Article
LanguageEnglish
Published Hungary 1976
Subjects
4-Chloromercuribenzenesulfonate - pharmacology
Cell Division
Edetic Acid - pharmacology
Peptide Hydrolases - metabolism
Phenylmethylsulfonyl Fluoride - pharmacology
Protease Inhibitors
Ribosomes - metabolism
Spores, Bacterial - enzymology
Streptomyces griseus - enzymology
Streptomyces griseus - growth & development
Streptomyces griseus - ultrastructure
Subcellular Fractions - enzymology
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Summary:Subcellular fractions were prepared from Streptomyces griseus No. 45-H at different stages of life cycle, and their proteolytic activity was examined. The highest proteolytic activity was found in the 24- and 72- h-old vegetative hyphae, the lowest in the resting spores. Spores contained about 9--30% of the proteolytic activity of vegetative cells. At the age of 16 h about 80%, at 26 h 70%, at 72 h 40%, and in spores about 60% of the proteolytic activity was particulate. The greatest part of the proteolytic activity could be inhibited by EDTA, lower levels of serine and sulfhydryl protease activities were detected in the cell-free extracts of vegetative cells.
ISSN:0001-5288