Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material

The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, contrasting with the rigid spherical appearance of elem...

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Bibliographic Details
Published inAnnales de microbiologie Vol. 129 B; no. 3; p. 313
Main Authors Popov, V, Eb, F, Lefebvre, J F, Orfila, J, Viron, A
Format Journal Article
LanguageEnglish
Published France 01.10.1978
Subjects
Animals
Bacteriological Techniques
Cattle
Cell Wall - analysis
Cell Wall - ultrastructure
Chick Embryo
Chlamydia trachomatis - ultrastructure
Chlamydophila psittaci - ultrastructure
Culture Media
DNA, Bacterial - isolation & purification
Edetic Acid
Female
Frozen Sections
Histocytochemistry
Humans
Microscopy, Electron
Microtomy
Ribonucleoproteins - isolation & purification
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Summary:The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, contrasting with the rigid spherical appearance of elementary bodies. Ultrastructural cytochemical study shows DNA fibrils dispersed throughout the cytoplasm in reticulate bodies whereas DNA is condensed in a nucleoid in elementary and intermediate bodies. The EDTA regressive technique reveals ribonucleoproteins in reticulate and elementary bodies of both studied strains.
ISSN:0300-5410