The modification of HLA-B27-positive lymphocytes by the culture filtrate of Klebsiella K43 BTS 1 is a metabolically active process

• Published in: Clinical and experimental immunology Vol. 62; no. 3; pp. 672 - 677
• Main Authors: SULLIVAN, J. S, GECZY, A. F
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell 01.12.1985
• Subjects: Antigens, Bacterial - immunology; Antimetabolites - pharmacology; Biological and medical sciences; Culture Media; Dose-Response Relationship, Immunologic; Filtration; Fundamental and applied biological sciences. Psychology; Fundamental immunology; HLA Antigens - analysis; HLA-B27 Antigen; Humans; Klebsiella - immunology; Lymphocytes - immunology; Lymphocytes - metabolism; Tissue, organ and graft immunology; Klebsiella; Modification; HLA-System; Dexamethasone; Major histocompatibility system; Gut; Diseases of the osteoarticular system; Filtrate; Metabolic inhibitor; Metabolism; Alloantigen; Microorganism culture; Bacteria; Inhibition; Lymphocyte; Klebsielleae; Ankylosing spondylitis; Enterobacteriaceae
• ISSN: 0009-9104; 1365-2249

The culture filtrate of some bacteria contains a modifying factor which specifically interacts with the cells of HLA-B27-positive normal individuals (BS+ AS-); this modification results in a serologically detectable change similar to that seen on the cells of patients with ankylosing spondylitis (B27+ AS+). Puromycin, 5-azacytidine, actinomycin D, cyclosporin A, dexamethasone, indomethacin, colchicine, quinacrine, chlorpromazine but not tunicamycin can effectively inhibit the in vitro modification of HLA-B27-positive lymphocytes from normal individuals. These studies suggest that a number of different metabolic pathways may be involved in the modification of B27+ AS- lymphocytes by certain enteric antigens.