Comparison of the drug metabolizing enzymes in the liver and kidneys from homozygous nude swiss, heterozygous normal swiss, homozygous normal Swiss and DBA/2 mice

The hepatic and renal microsomal drug metabolizing enzyme systems were isolated from homozygous nude Swiss (nu/nu), heterozygous normal Swiss (nu/+), homozygous normal Swiss (+/+) and DBA/2 mice. Microsomal protein and cytochrome P-450 concentrations were measured and the activity of ethylmorphine d...

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Bibliographic Details
Published inResearch communications in chemical pathology and pharmacology Vol. 15; no. 2; p. 267
Main Authors Freudenthal, R I, Leber, A P, Emmerling, D C, Kerchner, G A, Ovejera, A A
Format Journal Article
LanguageEnglish
Published United States 01.10.1976
Subjects
Animals
Body Weight - drug effects
Female
Kidney - drug effects
Kidney - enzymology
Liver - drug effects
Liver - enzymology
Mice
Mice, Inbred DBA
Mice, Nude
Mixed Function Oxygenases - metabolism
Organ Size - drug effects
Oxidoreductases - metabolism
Species Specificity
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Summary:The hepatic and renal microsomal drug metabolizing enzyme systems were isolated from homozygous nude Swiss (nu/nu), heterozygous normal Swiss (nu/+), homozygous normal Swiss (+/+) and DBA/2 mice. Microsomal protein and cytochrome P-450 concentrations were measured and the activity of ethylmorphine demethylase, aniline hydroxylase, aryl hydrocarbon hydroxylase and UDP-glucuronyl transferase were determined. Hepatic microsomes from both experimental groups carrying the nude gene were able to metabolize aniline and ethylmorphine more rapidly (20% and 36%, respectively) than the DBA/2 or Swiss homozygous normal mice. No difference between test groups was observed for hepatic aryl hydrocarbon hydroxylase or UDP-glucuronyl transferase activity. Kidney microsomes from mice carrying the nude gene had approximately twice the aryl hydrocarbon hydroxylase activity of the other two experimental groups. Renal mixed-function oxidase pathways measured for the homozygous nude mouse showed a higher overall rate of activity than the other three experimental groups. No significant difference in renal UDP-glucuronyl transferase was observed between mouse groups.
ISSN:0034-5164