A method for sequencing polymerase chain reaction products can be used to sequence Bacillus subtilis "miniprep" plasmid DNA
Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for pol...
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Published in | BioTechniques Vol. 10; no. 1; p. 17 |
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Main Author | |
Format | Journal Article |
Language | English |
Published |
England
01.01.1991
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Subjects | |
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Abstract | Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for polymerase chain reaction product sequencing, it was found that this protocol could be used for sequencing plasmid DNA isolated from Bacillus subtilis. |
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AbstractList | Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for polymerase chain reaction product sequencing, it was found that this protocol could be used for sequencing plasmid DNA isolated from Bacillus subtilis. |
Author | Bechhofer, D H |
Author_xml | – sequence: 1 givenname: D H surname: Bechhofer fullname: Bechhofer, D H organization: Department of Biochemistry, Mount Sinai School of Medicine, New York, NY 10029 |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/1900697$$D View this record in MEDLINE/PubMed |
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Snippet | Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to... |
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SubjectTerms | Bacillus subtilis - genetics Base Sequence DNA, Bacterial - genetics Electrophoresis, Polyacrylamide Gel Plasmids Polymerase Chain Reaction - methods |
Title | A method for sequencing polymerase chain reaction products can be used to sequence Bacillus subtilis "miniprep" plasmid DNA |
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