A method for sequencing polymerase chain reaction products can be used to sequence Bacillus subtilis "miniprep" plasmid DNA

Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for pol...

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Bibliographic Details
Published inBioTechniques Vol. 10; no. 1; p. 17
Main Author Bechhofer, D H
Format Journal Article
LanguageEnglish
Published England 01.01.1991
Subjects
Bacillus subtilis - genetics
Base Sequence
DNA, Bacterial - genetics
Electrophoresis, Polyacrylamide Gel
Plasmids
Polymerase Chain Reaction - methods
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Summary:Several methods for sequencing double-stranded plasmid DNA isolated from E. coli have been described. These methods are usually not effective when used to sequence plasmid DNA isolated from Bacillus subtilis. In the course of developing a simplified version of a previously published protocol for polymerase chain reaction product sequencing, it was found that this protocol could be used for sequencing plasmid DNA isolated from Bacillus subtilis.
ISSN:0736-6205