Karyometric marker features in fine needle aspirates of invasive follicular carcinoma of the thyroid

Karyometric measurements were performed on fine needle aspirates of clearly identifiable tumor areas and adjacent normal-appearing areas in the surgical specimens from ten patients with invasive follicular carcinoma of the thyroid. Similar measurements were performed on aspirates from nine patients...

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Bibliographic Details
Published inAnalytical and quantitative cytology and histology Vol. 12; no. 1; p. 35
Main Authors Galera-Davidson, H, Bartels, P H, Fernandez-Rodriguez, A, Dytch, H E, Lerma-Puertas, E, Bibbo, M
Format Journal Article
LanguageEnglish
Published United States 01.02.1990
Subjects
Adenocarcinoma - analysis
Adenocarcinoma - ultrastructure
Biomarkers, Tumor - analysis
Biopsy, Needle
Cell Nucleus - analysis
Coloring Agents
Humans
Image Processing, Computer-Assisted
Karyometry
Ploidies
Rosaniline Dyes
Staining and Labeling
Thyroid Neoplasms - analysis
Thyroid Neoplasms - ultrastructure
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Summary:Karyometric measurements were performed on fine needle aspirates of clearly identifiable tumor areas and adjacent normal-appearing areas in the surgical specimens from ten patients with invasive follicular carcinoma of the thyroid. Similar measurements were performed on aspirates from nine patients free of thyroid disease (controls). A total of 95 karyometric features were evaluated for each nucleus. Analysis of variance of optical density values indicated (1) a similarity between tumor and normal-appearing nuclei from carcinoma cases, (2) a significant difference between those nuclei and control nuclei and (3) that most of the differences were due to the differences of tissue origin. Stepwise linear discriminant analysis selected ten features that produced a statistically highly significant separation of tumor nuclei from control nuclei. A similar analysis selected six features that produced a statistically highly significant discrimination of normal-appearing nuclei from control nuclei; the validity of those karyometric features as markers of malignancy in normal-appearing nuclei from tissues adjacent to invasive follicular carcinomas of the thyroid was demonstrated by analysis in further training and control sets of nuclei. This analysis in thyroid aspirates identified more marker features than did a previous similar analysis using tissue sections.
ISSN:0884-6812