Development of sensitive methods for detection of latent Kilham virus infection of rats

The developed system of procedures, including preliminary selection of rats according to the presence of 19 S antibody in the blood serum, and the use of the most sensitive methods has increased considerably the incidence of detection of Kilham virus (KV) in organs of latently infected rats. To isol...

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Published inActa virologica (Anglickâa verze) Vol. 19; no. 4; p. 354
Main Authors Shalunova, N V, Dzagurov, S G, Gavrilov, K I, Petruchuk, E M, Gusarevich, E A, Uskov, V N, Babanova, T A
Format Journal Article
LanguageEnglish
Published Slovakia 01.07.1975
Subjects
Animals
Antibodies, Viral - isolation & purification
Cells, Cultured
Cytopathogenic Effect, Viral
Fluorescent Antibody Technique
Hemagglutinins, Viral - isolation & purification
Parvoviridae - isolation & purification
Parvovirus - immunology
Parvovirus - isolation & purification
Rats
Rodent Diseases - microbiology
Virus Diseases - veterinary
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Summary:The developed system of procedures, including preliminary selection of rats according to the presence of 19 S antibody in the blood serum, and the use of the most sensitive methods has increased considerably the incidence of detection of Kilham virus (KV) in organs of latently infected rats. To isolate and establish KV strains from the organs of rats in which the specific antigen was detected by immunofluorescence, at least 5-9 passages in rat embryo cell cultures or in newborn Syrian hamsters were required.
ISSN:0001-723X