Immunochemical studies on a Yersinia enterocolitica O:9 lipopolysaccharide cross-reacting with Brucella abortus and Vibrio cholerae extracts

The presence of two distinct lipopolysaccharides in Yersinia enterocolitica O:9 is described: one isolated from the aqueous phase and one from the phenol phase (Westphal system). The sugar moiety of the phenol phase lipopolysaccharide has been identified as being responsible for the serologic cross-...

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Bibliographic Details
Published inAnnales de microbiologie Vol. 129 B; no. 3; p. 425
Main Authors Sandulache, R, Marx, A
Format Journal Article
LanguageEnglish
Published France 01.10.1978
Subjects
Animals
Antigens, Bacterial - analysis
Brucella abortus - immunology
Cross Reactions - drug effects
Hemagglutination Tests
Humans
Lipid A - analysis
Lipopolysaccharides - isolation & purification
Lipopolysaccharides - pharmacology
Rabbits
Vibrio cholerae - immunology
Yersinia - immunology
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Summary:The presence of two distinct lipopolysaccharides in Yersinia enterocolitica O:9 is described: one isolated from the aqueous phase and one from the phenol phase (Westphal system). The sugar moiety of the phenol phase lipopolysaccharide has been identified as being responsible for the serologic cross-reaction of Y. enterocolitica O:9, Brucella abortus and Vibrio cholerae. The phenol phase antigen consists of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid, heptose, lipid A and a protein moiety. Haemagglutination experiments revealed two different structures: one that readily coats erythrocytes and another that requires alkali treatment. The former may be separated by repeated adsorptions on erythrocytes. Serologically, the two structures behave like a single antigen. The action of normal rabbit serum on the erythrocyte-containg capacity of the two structures was studied.
ISSN:0300-5410