Localization of bovine seminal plasma RNA-A BS1, on the surface of bovine spermatozoa

Using IgG antibodies raised against RNA-A BS1, the presence of this seminal RNA-A on the surface of bovine spermatozoa has been demonstrated. Indirect immunofluorescence and immunoferritin methods showed that this protein coats the surface of ejaculated bovine spermatozoa, but the pattern of binding...

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Bibliographic Details
Published inCellular and molecular biology Vol. 35; no. 3; p. 285
Main Authors Shivaji, S, Rukmini, V, Gupta, P D, Scheit, K H, Bhargava, P M
Format Journal Article
LanguageEnglish
Published England 1989
Subjects
Animals
Cattle
Endoribonucleases - analysis
Fluorescent Antibody Technique
Male
Microscopy, Electron
Sperm Head - enzymology
Sperm Head - ultrastructure
Sperm Tail - enzymology
Sperm Tail - ultrastructure
Spermatozoa - enzymology
Spermatozoa - ultrastructure
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Summary:Using IgG antibodies raised against RNA-A BS1, the presence of this seminal RNA-A on the surface of bovine spermatozoa has been demonstrated. Indirect immunofluorescence and immunoferritin methods showed that this protein coats the surface of ejaculated bovine spermatozoa, but the pattern of binding of the label varied from cell to cell. More than 50% of the spermatozoa showed labelling all over, except the anterior head region; about 30% showed labelling all over except the region below the equatorial plate region; and the remaining were either completely labelled or showed labelling only in the head or the tail region. The head-tail junction (the neck region) was not labelled in any case.