Histomorphology and small intestinal sodium-dependent glucose transporter 1 gene expression in piglets fed phytic acid and phytase-supplemented diets

• Published in: Journal of animal science Vol. 89; no. 8; pp. 2485 - 2490
• Main Authors: WOYENGO, T. A, RODRIGUEZ-LECOMPTE, J. C, ADEOLA, O, NYACHOTI, C. M
• Format: Journal Article
• Language: English
• Published: Champaign, IL American Society of Animal Science 01.08.2011
• Subjects: 6-Phytase - pharmacology; Animal Feed; Animal Nutritional Physiological Phenomena; Animal productions; Animals; Biological and medical sciences; Diet - veterinary; Dietary Supplements; Escherichia; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Intestine, Small - anatomy & histology; Intestine, Small - drug effects; Intestine, Small - metabolism; Male; Phytic Acid - pharmacology; Sodium-Glucose Transporter 1 - genetics; Sodium-Glucose Transporter 1 - metabolism; Swine - physiology; Terrestrial animal productions; Vertebrates; sodium-dependent glucose transporter 1 expression; 4-Phytase; Enzyme; Phosphoric monoester hydrolases; Esterases; Supplemented diet; Glucose; Gene expression; Phytic acid; Feeding; phytase; Sodium; Antinutrient factor; Hydrolases; Carbohydrate; piglet; histomorphology
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/jas.2010-3204

An experiment was conducted to determine the effect of dietary phytic acid (PA) and phytase supplementation on small intestinal histomorphology and Na-dependent glucose transporter 1 (SGLT1) gene expression in piglets. Twenty-four piglets with an average initial BW of 7.60 ± 0.73 kg were randomly assigned to 3 experimental diets, to give 8 piglets per diet. The diets were a casein-cornstarch-based diet that was supplemented with 0 or 2% PA, or 2% PA (as Na phytate) plus an Escherichia coli-derived phytase at 500 phytase units/kg. The basal diet was formulated to meet the 1998 NRC energy, digestible AA, mineral, and vitamin requirements for piglets. After 10 d of feeding, the piglets were killed to determine small intestinal histomorphology and small intestinal SGLT1 gene expression. Phytic acid supplementation did not affect (P > 0.1) villus height (VH) and the VH-to-crypt depth (CD) ratio, but did decrease (P < 0.05) CD in the jejunum. Phytase supplementation did not affect (P > 0.1) VH, CD, and the VH-to-CD ratio. Phytic acid supplementation reduced SGLT1 gene expression in the duodenum, jejunum, and ileum by 1.1-, 5.4-, and 2.4-fold, respectively. Phytase supplementation increased SGLT1 gene expression in the jejunum by 2.6-fold, but reduced SGLT1 gene expression in the duodenum and ileum by 2.0- and 4.0-fold, respectively. In conclusion, PA reduced CD in the jejunum and SGLT1 gene expression in the duodenum, jejunum, and ileum, whereas phytase supplementation increased the expression of SGLT1 in the jejunum. The reduced SGLT1 gene expression by PA implies that PA reduces nutrient utilization in pigs partly through reduced expression of SGLT1, which is involved in glucose and Na absorption. The increased expression of SGLT1 in the jejunum by phytase supplementation implies that phytase alleviated the negative effects of PA partly through increased expression of SGLT1.