Synergy between ajoene and ketoconazole in isolates of Microsporum canis. A preliminary study using fractional inhibitory concentration technique (FIC)

• Published in: Revista iberoamericana de micología Vol. 25; no. 3; pp. 157 - 162
• Main Authors: Ledezma, Eliades, Maniscalchi, Maria Teresa, Espinoza, Druvic Lemus
• Format: Journal Article
• Language: Spanish
• Published: Spain 30.09.2008
• Subjects: Antifungal Agents - pharmacology; Canis; Disulfides - pharmacology; Drug Synergism; Humans; Ketoconazole - pharmacology; Microbial Sensitivity Tests; Microsporum - drug effects; Microsporum - isolation & purification; Microsporum canis
• ISSN: 1130-1406
• DOI: 10.1016/S1130-1406(08)70037-8

Fungal infections are probably the most frequent infectious diseases affecting human being. Resistance to different anti-fungal drugs, and their bioavailability in the infection site, represent a problem for treatment. Looking for effective solutions, combination of two or more antifungal drugs to obtain an additive effect or synergic effect that potent antifungal activity has been investigated. In this study, the effect (additive, antagonist or synergistic) of ajoene and ketoconazole combination was evaluated in the growth and proliferation of filamentous fungi. Interactions in vitro were investigated in three isolates of Microsporum canis through a preliminary study using micro dilution, according to recommendations of NCCLS M-38A, with several modifications. Results obtained for CIF of each isolates studied (CIF = 0.18 0.36 microM), demonstrate that exists a very potent synergistic effect, when they are combined, and it represents a hope for future clinic trials to treat resilient fungal infections caused by M. canis.