Use of Surface Plasmon Resonance Technique for Studies of Inter-domain Interactions in Ion Channels

Ion channels are transmembrane proteins essential for cellular functions and are important drug targets. Surface plasmon resonance (SPR) is a powerful technique for investigating protein–protein and protein–small molecule ligand interactions. SPR has been underutilized for studies of ion channels, e...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2796; pp. 105 - 118
Main Authors Tiwari, Purushottam B., Kamgar-Dayhoff, Pareesa, Tiwari, Prakriti, McKillop, Maria I., Brelidze, Tinatin I.
Format Book Chapter Journal Article
LanguageEnglish
Published New York, NY Springer US 01.01.2024
SeriesMethods in Molecular Biology
Subjects
Animals
EAG
Ether-A-Go-Go Potassium Channels - chemistry
Ether-A-Go-Go Potassium Channels - metabolism
hERG
Humans
Ion channel function
Ion channel gating
Ion Channels - chemistry
Ion Channels - metabolism
KCNH
Kv10.1
Kv11.1
Ligands
Potassium channels
Protein Binding
Protein Interaction Domains and Motifs
SPR
Surface Plasmon Resonance - methods
Ion channel function
KCNH
hERG
SPR
Ion channel gating
EAG
Potassium channels
Kv10.1
Kv11.1
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Summary:Ion channels are transmembrane proteins essential for cellular functions and are important drug targets. Surface plasmon resonance (SPR) is a powerful technique for investigating protein–protein and protein–small molecule ligand interactions. SPR has been underutilized for studies of ion channels, even though it could provide a wealth of information on the mechanisms of ion channel regulation and aid in ion channel drug discovery. Here we provide a detailed description of the use of SPR technology for investigating inter-domain interactions in KCNH potassium-selective and voltage-gated ion channels.
ISBN:9781071638170
1071638173
ISSN:1064-3745
1940-6029
DOI:10.1007/978-1-0716-3818-7_7