PCR cloning and sequencing of the coding portion of the thioredoxin-encoding gene from Streptomyces aureofaciens BMK

The nucleotide sequence of the PCR-cloned coding portion of the thioredoxin-encoding gene from Streptomyces aureofaciens BMK was determined. The deduced 106-amino-acid sequence was compared with three other thioredoxins.

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Bibliographic Details
Published inGene Vol. 138; no. 1-2; pp. 263 - 264
Main Authors LABUDOVA, O, NEMETHOVA, M, TURNA, J, KOLLAROVA, M
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier 28.01.1994
Amsterdam
New York, NY
Subjects
Amino Acid Sequence
Anabaena - genetics
Base Sequence
Biological and medical sciences
Cloning, Molecular - methods
Corynebacterium - genetics
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Polymerase Chain Reaction - methods
Sequence Homology, Amino Acid
Streptomyces aureofaciens
Streptomyces aureofaciens - genetics
Thioredoxins - biosynthesis
Thioredoxins - genetics
Actinomycetales
Streptomycetaceae
Gene
Nucleotide sequence
Sulfoprotein
Bacteria
Actinomycetes
Molecular cloning
Thioredoxin
Aminoacid sequence
Structural analysis
Streptomyces aureofaciens
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Summary:The nucleotide sequence of the PCR-cloned coding portion of the thioredoxin-encoding gene from Streptomyces aureofaciens BMK was determined. The deduced 106-amino-acid sequence was compared with three other thioredoxins.
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ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(94)90822-2