Novobiocin induces positive supercoiling of small plasmids from halophilic archaebacteria in vivo

The halophilic archaebacterium Halobacterium strain GRB harbours a multicopy plasmid of 1.7 kb which is negatively supercoiled. After addition of novobiocin to culture medium all 1.7 kb plasmid molecules become positively supercoiled. Positive supercoiling occurs at the same dose of novobiocin inhib...

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Published inNucleic acids research Vol. 16; no. 4; pp. 1379 - 1391
Main Authors SIOUD, M, BALDACCI, G, DE RECONDO, A. M, FORTERRE, P
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.02.1988
Subjects
Archaebacteria
Biological and medical sciences
Conformational dynamics in molecular biology
DNA, Superhelical - drug effects
Fundamental and applied biological sciences. Psychology
Halobacterium
Halobacterium - genetics
Molecular biophysics
Novobiocin - pharmacology
Nucleic Acid Hybridization
Osmolar Concentration
Plasmids - drug effects
Plasmid
In vivo
Archaeobacteria
Halophily
Induction
Superhelical structure
Bacteria
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Summary:The halophilic archaebacterium Halobacterium strain GRB harbours a multicopy plasmid of 1.7 kb which is negatively supercoiled. After addition of novobiocin to culture medium all 1.7 kb plasmid molecules become positively supercoiled. Positive supercoiling occurs at the same dose of novobiocin inhibiting the eubacterial DNA gyrase in vitro. Novobiocin also induces positive supercoiling of pHV2, a 6.3 kb plasmid from Halobacterium volcanii. These results indicate the existence of a mechanism producing positive superturns in halobacteria. The 1.7 kb plasmid from Halobacterium GRB could be used to produce high amounts of pure positively supercoiled DNA for biophysical and biochemical studies.
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ISSN:0305-1048
1362-4962