The role of miR-155 in pathogenesis of immune thrombocytopenia

• Published in: Zhong hua yi xue za zhi Vol. 96; no. 14; p. 1103
• Main Authors: Guo, Y, Qu, W, Wang, Y H, Liu, H, Li, L J, Wang, H Q, Fu, R, Wu, Y H, Guan, J, Xing, L M, Shao, Z H
• Format: Journal Article
• Language: Chinese
• Published: China 12.04.2016
• Subjects: B-Lymphocytes; Case-Control Studies; Cell Count; Flow Cytometry; Gene Expression Regulation; Humans; Lymphocyte Count; MicroRNAs - blood; Platelet Count; Purpura, Thrombocytopenic, Idiopathic; Thrombocytopenia
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2016.14.008

To explore the role of microRNA-155 (miR-155) in pathogenesis of immune thrombocytopenia (ITP) through investigate the relevance between the expression of miR-155 in CD19(+) B cells in peripheral blood and the function of B lymphocytes in patients with ITP. A total of 55 ITP patients (30 newly diagnosed and 25 in remission) were collected from December 2014 to October 2015 in Tianjin Medical University General Hospital, and 25 healthy volunteers were recruited as controls. The CD19(+) B cells were extracted by immunomagnetic microbeads. The expression of miR-155 in CD19(+) B cells were detected by real-time fluorescent quantitative PCR. The levels of IgG, IgM and SH2 domain-containing inositol 5'-phosphatase 1 (SHIP-1) in CD19(+) B cells were measured by flow cytometry (FCM). Correlation between miR-155 and clinical parameters of ITP patients was analyzed. (1) The expression of miR-155 in CD19(+) B cells in newly diagnosed ITP patients (4.57±1.03) was significantly higher than that in remitted ITP patients (0