Isolation and characterization of thermophilic Campylobacter species from geese raised in Kars region (Turkey) using cultural, molecular and mass spectrometry methods

Thermophilic are found in the digestive tract of wild and domestic poultry and can be transmitted to humans following their fecal discharges. This study aimed to isolate thermophilic by culture from cloacal swabs of geese, commonly breeding in Kars region, and to identify the isolates by PCR and mas...

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Published inIranian journal of veterinary research Vol. 23; no. 1; pp. 24 - 31
Main Authors Demiroğlu, E G, Şahin, M, Büyük, F
Format Journal Article
LanguageEnglish
Published Iran School of Veterinary Medicine, University of Shiraz 2022
Subjects
Original
Thermophilic Campylobacter
MALDI-TOF MS
Goose
Antibiotic susceptibility
PCR
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Summary:Thermophilic are found in the digestive tract of wild and domestic poultry and can be transmitted to humans following their fecal discharges. This study aimed to isolate thermophilic by culture from cloacal swabs of geese, commonly breeding in Kars region, and to identify the isolates by PCR and mass spectrometry. Antibiotics susceptibility and resistance genes of the isolates were also analysed. The study included 400 cloacal swab samples of clinically healthy geese. The samples were cultured on mCCDA medium following the pre-enrichment in Preston broth. Identification of the isolates was performed by phenotypic methods, PCR, and MALDI-TOF MS. Antibiotic susceptibility and resistance genes of the isolates were analysed with the disc diffusion method and PCR, respectively. Thermophilic spp. were isolated from 157 (39.3%) samples. 151 (96.2%) isolates were identified and 6 (3.8%) by the phenotypic tests and PCR. Among 125 isolates analysed by MALDI-TOF MS, 119 (95.2%) were identified and 6 (4.8%) . The isolates' resistance to ampicillin, tetracycline, ciprofloxacin, gentamicin, and azithromycin were found 33.8%, 41.4%, 75.2%, 12.1%, and 7.6%, respectively. The distributions of , , , and genes were 3.2%, 90.8%, 50.8%, and 52.7%, respectively. Since geese are raised in pastures in the Kars region, protecting and not polluting the existing natural environment and preventing their contact with wild birds will prevent the spread of these microorganisms
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content type line 23
ISSN:1728-1997
2252-0589
DOI:10.22099/IJVR.2021.41103.5962