Comparative analysis between diatom nitric acid digestion method and plankton 16S rDNA PCR method

• Published in: Fa yi xue za zhi Vol. 29; no. 5; p. 356
• Main Authors: Han, Jun-ge, Wang, Cheng-bao, Li, Xing-biao, Fan, Yan-yan, Feng, Xiang-ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2013
• Subjects: Adolescent; Adult; Diatoms - classification; Diatoms - isolation & purification; DNA, Ribosomal - genetics; Drowning - diagnosis; Female; Forensic Medicine - methods; Fresh Water - analysis; Humans; Kidney; Liver; Lung; Male; Middle Aged; Nitric Acid; Plankton - genetics; Plankton - isolation & purification; Polymerase Chain Reaction - methods; RNA, Ribosomal, 16S - genetics; Young Adult
• ISSN: 1004-5619
• DOI: 10.3969/j.issn.1004-5619.2013.05.010

To compare and explore the application value of diatom nitric acid digestion method and plankton 16S rDNA PCR method for drowning identification. Forty drowning cases from 2010 to 2011 were collected from Department of Forensic Medicine of Wenzhou Medical University. Samples including lung, kidney, liver and field water from each case were tested with diatom nitric acid digestion method and plankton 16S rDNA PCR method, respectively. The Diatom nitric acid digestion method and plankton 16S rDNA PCR method required 20 g and 2 g of each organ, and 15 mL and 1.5 mL of field water, respectively. The inspection time and detection rate were compared between the two methods. Diatom nitric acid digestion method mainly detected two species of diatoms, Centriae and Pennatae, while plankton 16S rDNA PCR method amplified a length of 162 bp band. The average inspection time of each case of the Diatom nitric acid digestion method was (95.30 +/- 2.78) min less than (325.33 +/- 14.18) min of plankton 16S rDNA PCR method (P <