miR-501 promotes hemangioma progression by targeting HOXD10

• Published in: American journal of translational research Vol. 11; no. 4; pp. 2439 - 2446
• Main Authors: Zeng, Zhaofan, Liu, Sahua, Cai, Junhong, Li, Zhenzhen, Wu, Hongfei, Chen, Hao, Huang, Yanjing
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 01.01.2019
• Subjects: Original; cell behaviors; miR-501; hemangiomas; HOXD10; oncogenic miRNA
• ISSN: 1943-8141; 1943-8141

MicroRNAs (miRNAs) are often abnormally expressed in human cancers to act as either oncogenes or tumor suppressor genes. MiRNA-501 (miR-501) has been found to be abnormally expressed in certain types of cancer, but its expression and biological role in hemangioma remain to be fully elucidated. In this study, the expression of miR-501 in hemangioma cell lines was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The TargetScan algorithm, luciferase activity reporter assay, and Western blot analysis were conducted to validate homeobox D10 ( ) as a direct target of miR-501. The results revealed that miR-501 expression was upregulated in hemangioma cell lines. Downregulation of miR-501 inhibited hemangioma cell proliferation, cell cycle progression, colony formation, migration, and invasion . Bioinformatics analysis indicated that was a putative target of miR-501. In addition, in a luciferase reporter system, it was confirmed that is a direct target of miR-501. It was also demonstrated HOXD10 downregulation reversed the effects of the miR-501 inhibitor on hemangioma cell activities. These findings indicated that miR-501 targeted to promote hemangioma cell processes, suggesting that miR-501 has an oncogenic role in the pathogenesis of hemangioma.