miR-29a inhibits adhesion, migration, and invasion of osteosarcoma cells by suppressing CDC42

• Published in: International journal of clinical and experimental pathology Vol. 12; no. 11; pp. 4171 - 4180
• Main Authors: Liu, Zheng-Jie, Chen, Shun-Guang, Yang, Ye-Zi, Lu, Sheng-Jun, Zhao, Xun-Ming, Hu, Biao, Zhang, Ling
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 01.01.2019
• Subjects: Original; miRNA; bioinformatics analysis; expression profiling; osteosarcoma
• ISSN: 1936-2625

Osteosarcoma is one of the most common tumors of the bone in children and adolescents worldwide. The relapse and metastasis of osteosarcoma are a major therapeutic challenge. Recently, several metastasis regulators, including miRNAs, kinases, and lncRNAs, were reported in osteosarcoma. Identifying novel regulators of metastasis will be useful to explore novel biomarkers for osteosarcoma. The present study showed miR-29a overexpression significantly inhibited HOS and MG-63 cell adhesion, invasion, and migration. About 70% of the wound area was repaired by migrating cells after 24 h in the control group, and only 50% of the wound area was repaired in the miR-29a overexpression group. The numbers of invading cells were decreased by 40% and 50% in HOS and MG-63 cells transfected with miR-29a, respectively, compared with the negative control group. Moreover, the present study validated that CDC42 was a direct target of miR-29a in OS cells. In conclusion, miR-29a may serve as a therapeutic target for osteosarcoma.