Associations of sigmoid colon mucosal mast cells with bowel symptoms and psychological status in patients with irritable bowel syndrome with diarrhea

• Published in: Zhong hua yi xue za zhi Vol. 96; no. 30; p. 2398
• Main Authors: Xu, D, Chen, W, Zhou, W X, Wang, C D, Fei, G J, Zhu, L M, Xin, H W, Zhong, D R, Sun, G, Fang, X C
• Format: Journal Article
• Language: Chinese
• Published: China 09.08.2016
• Subjects: Abdominal Pain; Adult; Anxiety; Biopsy; Colon, Sigmoid; Comorbidity; Depression; Diarrhea; Humans; Intestinal Mucosa; Irritable Bowel Syndrome; Mast Cells; Psychiatric Status Rating Scales; Stress, Psychological; Surveys and Questionnaires
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2016.30.007

To investigate the bowel symptoms and psychological status of patients with irritable bowel syndrome (IBS) with diarrhea (IBS-D), and to verify whether sigmoid colon mucosal mast cells (MCs) and their activation have effect on the symptoms and psychological status of IBS-D patients. Patients meeting Rome Ⅲ diagnostic and subtyping criteria of IBS-D who visited the outpatient clinic of gastroenterology of Peking Union Medical College Hospital were consecutively enrolled between July 2009 and June 2012. IBS symptoms questionnaire was completed using face-to-face interview, and Hamilton Anxiety Scale (HAMA)/ Hamilton Depression Scale (HAMD) were administrated to evaluate psychological status, both by well-trained investigators. Mast cell tryptase monoclonal antibody was used for immunohistochemical staining to detect MCs and degranulated MCs in mucosal biopsy of sigmoid colon. MCs and degranulated MCs were blindly counted by a senior pathologist, and presented as number of cells in high power field (HPF) and per