Expression of Heme Oxygenase-1 and Leukemia Inhibitory Factor in Maternal Plasma and Placental Tissue in a Lipopolysaccharide-Induced Late Pregnancy Preterm Birth Mouse Model

• Published in: Journal of reproductive medicine Vol. 61; no. 1-2; p. 39
• Main Authors: Wang, Fan, Xiao, Mi, Lin, Xiao-Jie, Muhammad, Siddiq, Piao, Xian-Hua, Liu, Li
• Format: Journal Article
• Language: English
• Published: United States 01.01.2016
• Subjects: Animals; Disease Models, Animal; Female; Heme Oxygenase-1 - blood; Heme Oxygenase-1 - genetics; Leukemia Inhibitory Factor - blood; Leukemia Inhibitory Factor - genetics; Mice; Placenta - chemistry; Placenta - metabolism; Pregnancy; Premature Birth - blood; Premature Birth - genetics; Premature Birth - metabolism
• ISSN: 0024-7758

To investigate the expression of heme oxygenase-1 (HO-1) and leukemia inhibitory factor (LIF) in maternal plasma and placental tissue in intrauterine infection-induced preterm birth. Using a mouse model of intrauterine infection in preterm birth, we used magnetic beads to extract normal pregnant mouse spleen Treg cells, injecting them into lipopolysaccharide (LPS)-treated pregnant mice. The subjects were divided into 4 groups: control group, LPS group, LPS+PBS group, and LPS+Treg group. RT-PCR and Western blot were used to evaluate HO-1, LIF mRNA, and protein levels in the placenta. ELISA was used to detect these parameters in the peripheral blood of pregnant mice. The expression of HO-1 and LIF in the placenta of the LPS group was significantly decreased when compared to that of the control group (p<0.05). Serum HO-1 and LIF levels were higher than in the control group (p<0.05). In the Treg cell-treated group placental tissue HO-1 and LIF expression were significantly higher than in the LPS group (p<0.05), and serum HO-1 and LIF expression were significantly lower than in the LPS group (p<0.05). HO-1 and LIF participate with Treg cells in the maternal-fetal interface, producing a unique immune microenvironment.