The protective effect of cardamonin on the factors involved in delayed cerebral vasospasm in a rat model of subarachnoid hemorrhage

• Published in: International journal of clinical and experimental pathology Vol. 11; no. 12; pp. 5955 - 5961
• Main Authors: Ma, Yudong, Yu, Tianlei, Zhang, Yan, Yin, Yiheng, Zhao, Zhenyu, Yu, Xinguang, Yu, Yaoyu
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 01.01.2018
• Subjects: Original; apoptosis; delayed cerebral vascular vasospasm; α-smooth muscle actin; Akt; c-myc; Cardamonin
• ISSN: 1936-2625

Delayed cerebral vasospasms (DCVS) may affect the prognosis of patients after subarachnoid hemorrhage (SAH), but available preventive approaches are inefficient. The objective of this study was to explore the effects of cardamonin treatment on factors associated with the occurrence of DCVS after SAH. Rat models of SAH were created using the internal carotid artery puncture method. Rats were randomized into four groups: SAH (n = 10), SAH + vehicle (saline solution) group (n = 10), SAH + cardamonin group (n = 10), and a control (sham operation) group (n = 6). H&E staining was used to determine the wall thickness of the basilar artery. Immunohistochemistry was used to detect p-AKT and alpha smooth muscle actin (α-SMA). Immunofluorescence was used to detect the changes in C-myc expression. The TUNEL assay was used to detect apoptosis. Basilar artery wall thickness in the SAH + cardamonin and control groups were significantly lower than in the SAH group and SAH + vehicle groups (all P < 0.01). Apoptosis and the expression of p-AKT and C-myc in the SAH + cardamonin group were significantly lower than in the SAH and SAH + vehicle groups (P < 0.05), while α-SMA expression was higher than in the SAH and SAH + vehicle groups (P < 0.01). Cardamonin seems to alleviate cerebral vasospasms after SAH. These effects may involve the inhibition of p-AKT, C-myc expression and apoptosis, and the increase of α-SMA expression.