The expression changes in microRNA-132 in the lipopolysaccharide-induced inflammation of rat alveolar macrophages

• Published in: Zhonghua wei zhong bing ji jiu yi xue Vol. 26; no. 2; p. 80
• Main Authors: Fen, Liu, Rong, Jiang, Zhenguo, Zeng, Ning, Zhao, Liang, Xia, Cheng, Nie, Kejian, Qian
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2014
• Subjects: Animals; Cell Line; Inflammation - pathology; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Lipopolysaccharides - adverse effects; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - metabolism; MicroRNAs - metabolism; Rats; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 2095-4352
• DOI: 10.3760/cma.j.issn.2095-4352.2014.02.005

To observe the kinetic changes in microRNA-132 (miR-132) expression in rat alveolar macrophages after lipopolysaccharide (LPS)-induced inflammation, and to investigate initially on the role of miR-132 in alveolar macrophages inflammatory response. The rat alveolar macrophages NR8383 cultured without pyrogen in vitro were divided into blank control group and LPS (1 mg/L) stimulated 3, 6, 12, 24 hours groups. Culture supernatants and cell pellets were collected at each time point respectively. Enzyme-linked immunosorbent assay (ELISA) was used to assay the production changes in tumor necrosis factor-α (TNF-α), interleukins (IL-1β and IL-6) in the supernatant. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of miR-132 in the cells. After stimulating rat alveolar macrophages with LPS, the production of TNF-α (ng/L: 364.83 ± 46.29 vs. 34.07 ± 8.62, P<0.01), IL-1β (ng/L: 153.83 ± 43.67 vs. 32.33 ± 10.62, P<0.05) and IL-6 (ng/L: 183.85 ± 43.52 vs. 42.62 ± 11.21, P<0.05) were