Analysis of the resistance mechanism and homology of carbapenems-resistant Pseudomonas aeruginosa

• Published in: Zhonghua shao shang za zhi Vol. 30; no. 1; p. 15
• Main Authors: Liu, Yang, Deng, Qiong, Yu, Yang, Cao, Xianwei, Xu, Qunfei, Wan, Lagen
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2014
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; beta-Lactamases - genetics; Carbapenems - pharmacology; DNA, Bacterial; Drug Resistance, Bacterial; Humans; Microbial Sensitivity Tests; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - isolation & purification
• ISSN: 1009-2587
• DOI: 10.3760/cma.j.issn.1009-2587.2014.01.005

To study the resistance mechanism and homology of carbapenems-resistant Pseudomonas aeruginosa (PA). A total of 812 strains of PA (identified) were isolated from sputum, urine, blood, pus, and drainage of patients with burn, severe pneumonia, diabetes, chronic obstructive pneumonia, myocarditis, liver transplantation, or brainstem hemorrhage hospitalized from January to September 2012. Drug resistance of the 812 strains of PA to 15 antibiotics commonly used in clinic, including piperacillin, imipenem, etc., was tested using the automatic microorganism identifying and drug sensitivity analyzer. Among the carbapenems-resistant PA isolates, synergism test with imipenem-ethylene diamine tetraacetic acid (EDTA) and enhancement test with imipenem-EDTA and ceftazidime-EDTA were used to screen metallo-β-lactamase (MBL)-producing strains; modified Hodge test was used to screen strains producing Klebsiella pneumoniae carbapenemases (KPC); the carbapenemase gene, plasmid mediated quinolone resistant (PMQR) gene, and mob